De Novo Protein Sequencing

Hydrolyze monoclonal antibody proteins into short peptides by using multiple enzymes.

Using a high-precision mass spectrometer to generate tandem mass spectrometry data.

Using PEAKS AB de novo sequencing algorithm decomposes tandem mass spectrometry data into short peptide sequences to obtain amino acid sequence information.

Assemble amino acid sequences using the de novo algorithm to obtain complete antibody protein sequences, and identify leucine and isoleucine one by one.

Verify whether the molecular weight of the assembled amino acid sequence is correct through antibody light and heavy chain molecular weight experiments.