With 20 years of experience in monoclonal antibody research and development and a complete antibody production line, ClongeneBio can provide you with one-stop customized services from antigen design, animal immunization, fusion screening to antibody production, and ultimately deliver high-quality monoclonal antibodies aligned with the client’s application requirements, and meeting clients’ different needs.
- Customer provision or ClongeneBio preparation according to customer requirements
- 4-5 Balb/C mice
- Detection of serum titer in mice
- Electrofusion
- Clone screening
- Preparation of ascites by intraperitoneal inoculation
- Octanoic acid-ammonium sulfate method
- ProteinA/G method
Antigen preparation
Animal immunization
Cell fusion
Subclonal screening
Antibody preparation
Antibody purification
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Preparation of NP monoclonal antibody by using hybridoma technique
The NP monoclonal antibody is prepared by hybridoma technique and purified by Protein A. The purified SDS-PAGE is shown in Figure 1. Its activity is analyzed by ELISA, and the result is shown in Figure 2. It can be seen from the results that NP antibody has high sensitivity and good activity.
