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Hybridoma Monoclonal Antibody Development

Hybridoma Monoclonal Antibody Development

With 20 years of experience in monoclonal antibody research and development and a complete antibody production line, ClongeneBio can provide you with one-stop customized services from antigen design, animal immunization, fusion screening to antibody production, and ultimately deliver high-quality monoclonal antibodies aligned with the client’s application requirements, and meeting clients’ different needs.


Service Details
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Antigen preparation (optional)

- Customer provision or ClongeneBio preparation according to customer requirements

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Animal immunization

- 4-5 Balb/C mice
- Detection of serum titer in mice

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Cell fusion and screening

- Electrofusion
- Clone screening

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Antibody preparation

- Preparation of ascites by intraperitoneal inoculation

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Antibody purification

- Octanoic acid-ammonium sulfate method 
- ProteinA/G method

Technology Process
Alternate Text Antigen preparation
Alternate Text Animal immunization
Alternate Text Cell fusion
Alternate Text Subclonal screening
Alternate Text Antibody preparation
Alternate Text Antibody purification
Service Workflow
Alternate Text Customer Consultation
Alternate Text Program and contract confirmation
Alternate Text Project initiation
Alternate Text Feedback and sample delivery
Alternate Text Scalable production
Alternate Text Product delivery
Case Study

Case #1. Preparation of NP monoclonal antibody by using hybridoma technique
The NP monoclonal antibody is prepared by hybridoma technique and purified by Protein A. The purified SDS-PAGE is shown in Figure 1. Its activity is analyzed by ELISA, and the result is shown in Figure 2. It can be seen from the results that NP antibody has high sensitivity and good activity.

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