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E.coli Protein Expression

E.coli Protein Expression

E.coli protein expression system is the most widely used protein expression system. Serves as an attractive host system due to its well-characterized genetic background, high yield capacity, robust production cycle, strong anti-pollution abilities, and ease of scaling, suitable for protein expression of many genera. However, the E.coli protein expression system also faces some limitations, such as hard to express, formation of inclusion bodies and endotoxin. As a leading protein antibody service supplier, ClongeneBio has more than ten years of experience in protein production and can solve various problems in the process of protein expression to meet the needs of customers.


Service Details
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Gene synthesis and subclone

- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation

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Expression identification and solubility analysis

- 10ml culture
- Expression conditions groping
- Protein expression evaluation

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Protein expression and purification

- Protein purification
- Protein denaturation and renaturation purification
- QC analysis

Technology Process
Alternate Text Gene synthesis
Alternate Text Vector construction and plasmid amplification
Alternate Text Pilot expression and purification study
Alternate Text Feedback pilot expression and purification study results
Alternate Text Protein expression amplification and purification
Alternate Text Product delivery
Service Workflow
Alternate Text Customer Consultation
Alternate Text Program and contract confirmation
Alternate Text Project initiation
Alternate Text Feedback and sample delivery
Alternate Text Scalable production
Alternate Text Product delivery
Case Study

Case #1. Express the target protein with His-tag in E.coli. 

According to the customer's requirements, the target protein is expressed in E.coli, and purified by Ni-NTA affinity chromatography, the SDS-PAGE is shown in Figure 1 and Figure 2.

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