E.coli protein expression system is the most widely used protein expression system. Serves as an attractive host system due to its well-characterized genetic background, high yield capacity, robust production cycle, strong anti-pollution abilities, and ease of scaling, suitable for protein expression of many genera. However, the E.coli protein expression system also faces some limitations, such as hard to express, formation of inclusion bodies and endotoxin. As a leading protein antibody service supplier, ClongeneBio has more than ten years of experience in protein production and can solve various problems in the process of protein expression to meet the needs of customers.
- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation
- 10ml culture
- Expression conditions groping
- Protein expression evaluation
- Protein purification
- Protein denaturation and renaturation purification
- QC analysis
Gene synthesis
Vector construction and plasmid amplification
Pilot expression and purification study
Feedback pilot expression and purification study results
Protein expression amplification and purification
Product delivery
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Express the target protein with His-tag in E.coli.
According to the customer's requirements, the target protein is expressed in E.coli, and purified by Ni-NTA affinity chromatography, the SDS-PAGE is shown in Figure 1 and Figure 2.
