The Beacon single-cell optofluidic system introduced by ClongeneBio facilitates the development of antibody drugs, tumor immunity and other cell biology research. It can introduce tens of thousands of single cells into nano upgraded micro-chamber for single cell isolation, detection, culture and export of integrated operation. ClongeneBio can obtain positive monoclonal antibodies within 35 days by using Beacon single-cell optofluidic system, which greatly improves the efficiency of antibody screening, thus speeding up the process of antibody drug discovery.
- 3-5 Balb/C mice were immunized
- Detection of serum titer in mice
- Plasma cells from mouse spleen were extracted
- Use Beacon for separation plasma cells
- Extract mRNA, reverse transcription, amplification heavy/light chain sequences
- Recombinant expression antibody
- Antibody purification
- Antibody to verify
- Five ELISA positive clones, each clone had 2 tubes of frozen cells
- 1 mg purified antibody
- COA
Immunization
Plasma B cells extraction
Beacon sorting
mRNA extraction and cDNA synthesis
Heavy & light chain amplification
Vector construction
Recombinant antibody expression and purification
Antibody validation
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Screening of single positive cells by using beacon single cell optofluidic systems
The Beacon single cell optofluidic systems is used to screen single positive cells, which can complete the whole screening process in only one day. Figure 1 shows the screening of positive cells, the positive cells are displayed in the form of fluorescence.
