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Beacon Single B Cell Screening

Beacon Single B Cell Screening

The Beacon single-cell optofluidic system introduced by ClongeneBio facilitates the development of antibody drugs, tumor immunity and other cell biology research. It can introduce tens of thousands of single cells into nano upgraded micro-chamber for single cell isolation, detection, culture and export of integrated operation. ClongeneBio can obtain positive monoclonal antibodies within 35 days by using Beacon single-cell optofluidic system, which greatly improves the efficiency of antibody screening, thus speeding up the process of antibody drug discovery.


Service Details
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Animal immunization


- 3-5 Balb/C mice were immunized
- Detection of serum titer in mice

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Plasma cell extraction and Beacon sorting


- Plasma cells from mouse spleen were extracted
- Use Beacon for separation plasma cells
- Extract mRNA, reverse transcription, amplification heavy/light chain sequences


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Recombinant antibody expression and verification


- Recombinant expression antibody
- Antibody purification
- Antibody to verify

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- Five ELISA positive clones, each clone had 2 tubes of frozen cells
- 1 mg purified antibody

Technology Process
Alternate Text Immunization
Alternate Text Plasma B cells extraction
Alternate Text Beacon sorting
Alternate Text mRNA extraction and cDNA synthesis
Alternate Text Heavy & light chain amplification
Alternate Text Vector construction
Alternate Text Recombinant antibody expression and purification
Alternate Text Antibody validation
Service Workflow
Alternate Text Customer Consultation
Alternate Text Program and contract confirmation
Alternate Text Project initiation
Alternate Text Feedback and sample delivery
Alternate Text Scalable production
Alternate Text Product delivery
Case Study

Case #1. Screening of single positive cells by using beacon single cell optofluidic systems
The Beacon single cell optofluidic systems is used to screen single positive cells, which can complete  the whole screening process in only one day. Figure 1 shows the screening of positive cells, the positive  cells are displayed in the form of fluorescence.

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