Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. Compared to the E.coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. ClongeneBio can provide high-quality experimental scheme and significantly improve the yield of your target protein，including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.
- Codon optimization and gene synthesis
- Clone the target gene into the vector plasmid
- Subcloning the target gene into a baculovirus transfer vector
- Transfect insect cells, prepare viral stocks and expand cultures
- Pilot expression and purification trial
- QC analysis
- Target protein
- QC analysis
Gene synthesis
Clone the target gene into the vector plasmid
Cloning the target gene into the baculovirus transfer vector by transposition
Transfection of insect cells and virus expansion
Small-scale pilot expression and purification
Large -scale expression and purification (optional)
Product delivery
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Express the target protein in Baculovirus-Insect expression system
The target protein is expressed by Baculovirus-Insect expression system, the purified protein and quality test report are successfully delivered. The relevant SDS-PAGE and SEC-HPLC analysis are shown in Figure 1 and Figure 2.
