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Baculovirus-Insect Cell Protein Expression

Baculovirus-Insect Cell Protein Expression

Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. Compared to the E.coli expression system, insect expression system is superior in improving protein solubility, post-translational modification of protein and producing high expression secreted protein. ClongeneBio can provide high-quality experimental scheme and significantly improve the yield of your target protein,including the preparation of recombinant baculovirus, the expression and purification of recombinant protein and its complex.


Service Details
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Gene synthesis and subclone

- Codon optimization and gene synthesis
- Clone the target gene into the vector plasmid

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Construction of recombinant baculovirus vector

- Subcloning the target gene into a baculovirus transfer vector

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Recombinant baculovirus expansion culture and protein expression & purification

- Transfect insect cells, prepare viral stocks and expand cultures 
- Pilot expression and purification trial 
- QC analysis

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Scale up expression and purification (optional)

- Target protein
- QC analysis

Technology Process
Alternate Text Gene synthesis
Alternate Text Clone the target gene into the vector plasmid
Alternate Text Cloning the target gene into the baculovirus transfer vector by transposition
Alternate Text Transfection of insect cells and virus expansion
Alternate Text Small-scale pilot expression and purification
Alternate Text Large -scale expression and purification (optional)
Alternate Text Product delivery
Service Workflow
Alternate Text Customer Consultation
Alternate Text Program and contract confirmation
Alternate Text Project initiation
Alternate Text Feedback and sample delivery
Alternate Text Scalable production
Alternate Text Product delivery
Case Study

Case #1. Express the target protein in Baculovirus-Insect expression system

The target protein is expressed by Baculovirus-Insect expression system, the purified protein and quality  test report are successfully delivered. The relevant SDS-PAGE and SEC-HPLC analysis are shown in  Figure 1 and Figure 2.

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