The mammalian cell expression has the advantage of guiding the correct folding of protein, providing complex glycosylation modification. Therefore, the expressed recombinant protein is closest to natural higher biological protein molecules in molecular structure, physicochemical properties and biological function, and has better activity than prokaryotic expression system and eukaryotic expression system such as yeast and insect cell. HEK293 cells and CHO cells were used as expression hosts in the mammalian transient expression system of ClongeneBio. With years of experience in protein expression, ClongeneBio can provide flexible services to meet the needs of different customers.
- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation
- Transient transfection of HEK293/CHO cells
- Protein purification
- QC analysis
- Target protein
- QC analysis
Gene synthesis
Vector construction and plasmid amplification
Protein expression and purification
Large-scale expression and purification (optional)
Product delivery
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1: Transient expression of the target protein in mammalian cells
The mammalian transient expression system is used to express target protein. The SDS-PAGE of the purified protein is shown in Figure 1.
