ClongeneBio has developed a large-scale recombinant antibody production service, providing customers with recombinant antibodies in the amount of gram to kilogram. ClongeneBio designs the protocol according to the scale specified by the customer, optimizes the sequence design, culture protocol, transfection method and cell line construction protocol to meet customer's specific needs, and guarantees the delivery of the antibody of the expression amount and quality of customer's choice.
- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation
- Transfection of mammalian cells
- Small scale pilot expression and purification
- QC analysis
- Large-scale plasmid preparation
- Transient transfection and purification
- QC analysis
Gene synthesis
Vector construction and plasmid amplification
Protein pilot expression and purification
Sample evaluation
Large-scale protein expression and purification
Product delivery
Program and contract confirmation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Large-scale expression of recombinant antibody
The recombinant antibody was produced on a large scale by a 250L bioreactor, the SDS-PAGE and SEC-HPLC analysis results were shown in Figure 1 and Figure 2.
