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Large-scale Antibody Production Service

Large-scale Antibody Production Service

ClongeneBio has developed a large-scale recombinant antibody production service, providing customers with recombinant antibodies in the amount of gram to kilogram. ClongeneBio designs the protocol according to the scale specified by the customer, optimizes the sequence design, culture protocol, transfection method and cell line construction protocol to meet customer's specific needs, and guarantees the delivery of the antibody of the expression amount and quality of customer's choice.

 

Service Details
Alternate Text
Gene synthesis and subclone

 

- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation

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Small scale pilot expression and purification

 

- Transfection of mammalian cells
- Small scale pilot expression and purification
- QC analysis

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Large-scale production (up to 250L)

 

- Large-scale plasmid preparation
- Transient transfection and purification
- QC analysis 

Technology Process
Alternate Text Gene synthesis
Alternate Text Vector construction and plasmid amplification
Alternate Text Protein pilot expression and purification
Alternate Text Sample evaluation
Alternate Text Large-scale protein expression and purification
Alternate Text Product delivery
Service Workflow
Alternate Text Program and contract confirmation
Alternate Text Program and contract confirmation
Alternate Text Project initiation
Alternate Text Feedback and sample delivery
Alternate Text Scalable production
Alternate Text Product delivery
Case Study

Case #1. Large-scale expression of recombinant antibody
The recombinant antibody was produced on a large scale by a 250L bioreactor, the SDS-PAGE and SEC-HPLC  analysis results were shown in Figure 1 and Figure 2.

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